- ￥3120
- biorbyt
- orb182869
- 英国
- 2025年12月02日
- FC, IF, IHC-Fr, IHC-P
- Rabbit
- Human
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- 详细信息
- 技术资料
- 抗体名：
C5a anaphylatoxin Rabbit Polyclonal Antibody抗体
- 抗体英文名：
C5a anaphylatoxin Rabbit Polyclonal Antibody
- 靶点：
C5
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb182869
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human C5a anaphylatoxin (678-751/1676aa)
- 规格：
50 ul
产品描述：C5a anaphylatoxin Rabbit Polyclonal Antibody
别名:Anaphylatoxin C5a; C5; Complement C5; Complement C5 precursor; Complement component 5; CPAMD4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain; CO5_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human C5a anaphylatoxin (678-751/1676aa)
分子量:8/109 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P01031
Note:For research use only.
别名:Anaphylatoxin C5a; C5; Complement C5; Complement C5 precursor; Complement component 5; CPAMD4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain; CO5_HUMAN.
免疫原：KLH conjugated synthetic peptide derived from human C5a anaphylatoxin (678-751/1676aa)
分子量:8/109 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P01031
Note:For research use only.
Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-C5a anaphylatoxin antibody (orb182869), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Fixative-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Complement C5 beta chain) Polyclonal Antibody, Unconjugated (orb182869) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/Cell: human hepatoma tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-C5a anaphylatoxin Polyclonal Antibody, Unconjugated (orb182869) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human hepatoma tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-C5a anaphylatoxin Polyclonal Antibody, Unconjugated (orb182869) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
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C5a anaphylatoxin Rabbit Polyclonal Antibody抗体，orb182869，biorbyt
￥3120
