ARID1A Rabbit Polyclonal Antibody 抗体,orb316548,Biorbyt产品图

ARID1A Rabbit Polyclonal Antib

ody 抗体,orb316548,Biorbyt
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  • ¥5499
  • Biorbyt已认证
  • orb316548
  • 英国
  • 2026年07月22日
  • FC, ICC, IF, WB
  • Rabbit
  • Human, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      ARID1A Rabbit Polyclonal Antibody 抗体

    • 抗体英文名:

      ARID1A Rabbit Polyclonal Antibody

    • 靶点:

      AT-rich interactive domain-containing protein 1A

    • 浓度:

      Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

    • 应用范围:

      FC, ICC, IF, WB

    • 宿主:

      Rabbit

    • 适应物种:

      Human, Mouse

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb316548

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 形态:

      Lyophilized

    • 亚型:

      Rabbit IgG

    • 免疫原:

      详询

    • 规格:

      100 ug

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Anti-ARID1A Antibody. Tested in WB applications. This antibody reacts with Human.

    别名: AT-rich interactive domain-containing protein 1A; ARID domain-containing protein 1A; B120; BRG1-associated factor 250; BAF250; BRG1-associated factor 250a; BAF250A; Osa homolog 1; hOSA1; SWI-like protein; SWI/SNF complex protein p270; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1; hELD; ARID1A; BAF250, BAF250A, C1orf4, OSA1, SMARCF1

    应用稀释比例: Western blot, 0.1-0.5μg/ml, Human, Mouse Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x10^6 cells, Human

    免疫原: A synthetic peptide corresponding to a sequence in the middle region of human ARID1A, identical to the related mouse sequence.

    交叉反应: No cross-reactivity with other proteins

    分子量: 250-270 kDa

    防腐剂: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    纯化方法: Immunogen affinity purified.

    复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

    特异性: No cross reactivity with other proteins.

    研究领域: Cancer Biology, Epigenetics & Chromatin, Signal Transduction

    UniProt ID: O14497

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of ARID1A using anti-ARID1A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: SW620 Whole Cell Lysate at 40 ug. Lane 2: HEPG2 Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID1A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARID1A at approximately 242 kDa. The expected band size for ARID1A is at 242 kDa.

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    图标文献和实验
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