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Phospho-AMPK alpha-2 (Thr172)

Rabbit Polyclonal Antibody抗体,orb99303,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb99303
  • 英国
  • 2025年12月03日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody

    • 靶点

      PRKAA2

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb99303

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      参考产品说明书

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 2 around the phosphorylation site of Thr172 LR(p-T)SC

    • 规格

      50 ul

    产品描述:Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody

    别名:AMPK alpha 1(phospho T183)+AMPK alpha 2(phospho T172); PRKAA1(phospho T172); AMPK alpha 1+AMPK alpha 2(phospho T172); phospho-AMPK alpha-1(Thr183); 5'-AMP-activated protein kinase catalytic subunit alpha-2; AAPK2_HUMAN; AAPK1_HUMAN; ACACA kinase; Acetyl-CoA carboxylase kinase; AMPK alpha 2 chain; AMPK subunit alpha-2; AMPK2; AMPK 2; AMPKa2; AMPK a2; AMPK-a2AMPKalpha2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; PRKAA; PRKAA2; Protein kinase AMP activated alpha 2 catalytic subunit; Protein kinase AMP activated catalytic subunit alpha 2.

    免疫原:KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 2 around the phosphorylation site of Thr172 LR(p-T)SC

    预测反应性:Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep

    分子量:64 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:34447468

    UniProt ID:P54646

    Note:For research use only.

    产品细节图片1
    paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片2
    Fixative-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

    产品细节图片3
    Fixative-fixed, paraffin embedded (mouse heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片4
    Fixative-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片5
    Fixative-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片6
    Fixative-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-AMPK alpha-2 (Thr172)) Polyclonal Antibody, Unconjugated (orb99303) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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    图标文献和实验
    该产品被引用文献
    Melo B.P. 1 , Zacarias A.C. 1 , Oliveira J.C.C. 1 , Cordeiro L.M.S. 2 , Horta N.A.C. 3 , Poletini M.O. 3 , Tonoli C. 4 , Dos Santos M.L. 5 , Wanner S.P. 1 , Meeusen R. 8 , Heyman E. 6 7 , Avelar G.F. 5 , Soares D.D Enhancing Metabolic and Inflammatory Status in Insulin-Resistant Rats: Acute Intervention with Cocoa Flavanols and Submaximal Aerobic Exercise Activates Intracellular Signaling Pathways for Glucose Metabolism Clinical Nutrition, (2024), Li, Weilong et al. Emodin inhibits the proliferation of papillary thyroid carcinoma by activating AMPK Exp Ther Med, 22, 1075 (2021)
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    Phospho-AMPK alpha-2 (Thr172) Rabbit Polyclonal Antibody抗体,orb99303,biorbyt
    ¥3120