AKT3 Antibody (FITC) 抗体,orb344535,Biorbyt产品图

AKT3 Antibody (FITC) 抗体,orb344

535,Biorbyt
收藏
  • ¥10764
  • Biorbyt已认证
  • orb344535
  • 英国
  • 2026年08月05日
  • DOT, ELISA
  • Mouse
  • Human, Mouse, Rat
avatar
品牌商
13 年钻石会员
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      AKT3 Antibody (FITC) 抗体

    • 抗体英文名:

      AKT3 Antibody (FITC)

    • 靶点:

      AKT3

    • 浓度:

      1.0 mg/mL

    • 应用范围:

      DOT, ELISA

    • 宿主:

      Mouse

    • 适应物种:

      Human, Mouse, Rat

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb344535

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      FITC

    • 克隆性:

      Monoclonal

    • 保存条件:

      详询

    • 形态:

      Lyophilized

    • 亚型:

      IgG1

    • 免疫原:

      详询

    • 规格:

      50 ug

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: AKT3 FITC antibody (FITC)

    克隆号: 25F6.F6.D8

    别名: Mouse anti-AKT3 antibody FITC conjugation, fluorescein conjugated Mouse anti-AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

    应用稀释比例: ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IF: User Optimized, WB: User Optimized

    应用说明: Anti-AKT3 FITC Antibody has been tested by ELISA and dot blot and is suitable for Flow Cytometry, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

    免疫原: Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein.

    防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

    纯度: Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from tissue culture by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.

    研究领域: Signal Transduction

    UniProt ID: Q9Y243

    NCBI 参考序列: NP_001193658.1

    保存条件: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

    备注: For research use only.

    产品细节图片1
    Dot Blot of Mouse anti-AKT3 Monoclonal Antibody Fluorescein Conjugated. Antigen: His-tagged AKT3. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Mouse anti-AKT3 Monoclonal Antibody Fluorescein Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 1 HR at RT.

    产品细节图片2
    Immunohistochemistry of Mouse Anti-AKT3 antibody. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT-3 antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10000 for 1 h at RT. Localization: AKT3 is nuclear and occasionally cytoplasmic. Staining: AKT3 as precipitated brown signal with hematoxylin purple nuclear counterstain.

    产品细节图片3
    Immunohistochemistry of Mouse Monoclonal anti AKT3 Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH6.2. Pressure Cooker. Primary antibody: anti-AKT3 at 2 ug/ml for 1.5 hour @ room Temp. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45" RT.

    产品细节图片4
    Western Blot of Mouse Anti-AKT3 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.

    产品细节图片5
    Western Blot of Mouse anti-AKT3 antibody. Lane 1: Control. Lane 2: Rapa. Lane 3: T50. Lane 4: T250. Lane 5: Control. Lane 6: Rapa. Lane 7: T50. Lane 8: T250. Lane 9: AKT3 null. Load: 35 µg per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.

    产品细节图片6
    Western Blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验
    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody Conjugation Protocol

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • secondary antibody review -- data from 99 publications

      localization of tagged SAX-3 in 293T cell Molecular Probes 2       Alexa Fluor 647 immunocytochemistry   localization of BCG phagosomes   3       Alexa Fluor 647 immunohistochemistry   detect surface DFz2   3       FITC immunohistochemistry   determine

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    ¥0
    武汉博欧特生物科技有限公司
    2026年08月07日询价
    ¥4284
    深圳欣博盛生物科技有限公司
    2025年07月15日询价
    ¥1280
    湖北艾普蒂生物工程有限公司
    2025年07月12日询价
    询价
    美国CST中国公司(丁香通商铺)
    2026年10月05日询价
    询价
    上海邦景实业有限公司
    2025年07月08日询价
    AKT3 Antibody (FITC) 抗体,orb344535,Biorbyt
    ¥10764