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- 文献和实验
- 技术资料
- 抗体名:
AKT3 Antibody (FITC) 抗体
- 抗体英文名:
AKT3 Antibody (FITC)
- 靶点:
AKT3
- 浓度:
1.0 mg/mL
- 应用范围:
DOT, ELISA
- 宿主:
Mouse
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb344535
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
FITC
- 克隆性:
Monoclonal
- 保存条件:
详询
- 形态:
Lyophilized
- 亚型:
IgG1
- 免疫原:
详询
- 规格:
50 ug
产品子类型: Primary Antibody
描述: AKT3 FITC antibody (FITC)
克隆号: 25F6.F6.D8
别名: Mouse anti-AKT3 antibody FITC conjugation, fluorescein conjugated Mouse anti-AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma
应用稀释比例: ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IF: User Optimized, WB: User Optimized
应用说明: Anti-AKT3 FITC Antibody has been tested by ELISA and dot blot and is suitable for Flow Cytometry, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
免疫原: Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein.
防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
纯度: Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from tissue culture by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
研究领域: Signal Transduction
UniProt ID: Q9Y243
NCBI 参考序列: NP_001193658.1
保存条件: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
备注: For research use only.






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文献和实验8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
secondary antibody review -- data from 99 publications
localization of tagged SAX-3 in 293T cell Molecular Probes 2 Alexa Fluor 647 immunocytochemistry localization of BCG phagosomes 3 Alexa Fluor 647 immunohistochemistry detect surface DFz2 3 FITC immunohistochemistry determine
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