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NALP3/CIAS1 Rabbit Polyclonal

Antibody抗体,orb182473,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb182473
  • 英国
  • 2025年12月02日
  • FC
  • Rabbit
  • Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      NALP3/CIAS1 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      NALP3/CIAS1 Rabbit Polyclonal Antibody

    • 靶点

      NLRP3

    • 浓度

      1mg/ml

    • 应用范围

      FC

    • 宿主

      Rabbit

    • 适应物种

      Mouse

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb182473

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human Cryopyrin (551-630/1036aa)

    • 规格

      50 ul

    产品描述:NALP3/CIAS1 Rabbit Polyclonal Antibody

    别名:LRR and PYD domains-containing protein 3; AGTAVPRL; AII/AVP antibody Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLRP3; PYPAF 1; PYPAF1 antibody PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1.

    免疫原:KLH conjugated synthetic peptide derived from human Cryopyrin (551-630/1036aa)

    预测反应性:Human, Rat

    分子量:114 kDa

    应用稀释比例:Flow-Cyt=1ug/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:Q96P20

    Note:For research use only.

    产品细节图片1
    Blank control (black line): Raw264.7. Primary Antibody (green line): Rabbit Anti-NALP3/CIAS1 antibody (orb182473), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Sample: Lung (Rat) Lysate at 40 ug, Colon carcinoma (Human) Lysate at 40 ug, Primary: Anti-NALP3/CIAS1 (orb182473) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 114 kD, Observed band size: 114 kD.

    产品细节图片3
    Tissue/Cell: Rat tracheal tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cryopyrin Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
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