- ￥10296
- Biorbyt
- orb345931
- 英国
- 2026年08月05日
- DOT, ELISA
- Goat
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- 文献和实验
- 技术资料
- 抗体名：
Fab Biotin Antibody 抗体
- 抗体英文名：
Fab Biotin Antibody
- 浓度：
1.0 mg/mL
- 应用范围：
DOT, ELISA
- 宿主：
Goat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb345931
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Liquid (sterile filtered)
- 亚型：
IgG Fab
- 免疫原：
详询
- 规格：
1 mg
产品子类型: Primary Antibody
描述: Fab antibody
别名: Goat Fab Anti-Biotin Antibody, Goat Fab Fragment Anti-Biotin Antibody
应用稀释比例: ELISA: 1:5,000
应用说明: Fab Anti-Biotin Antibody has been tested by ELISA, dot blot, and SDS-PAGE and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user.
免疫原: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
纯度: Fab fragment of Anti-Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-papain or anti-Goat IgG F(c).
研究领域: Epigenetics & Chromatin
保存条件: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
备注: For research use only.
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文献和实验35 Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells
(1 -7 ). Production of selected Abs in sufficient quantity for further evaluation often requires the transfer of their coding sequences to vectors specfiically designed for expression in eukaryotic cells. For Fab and/or immunoglobulin G (IgG) expression, these systems
Phage-Display Libraries of Murine and Human Antibody Fab Fragments
This chapter describes efficient procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. Phagemid vectors are used for placing
Production of Human Fab Antibody Fragments from Phage Display Libraries
with the desired binding properties (1 ) (see review in ref. 2 ). Initially, the system was used for mapping antibody epitopes by expression of large libraries (107 –108 ) of random peptides (3 –5 ). However, the approach has proved readily applicable
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