- ￥5175
- Agrisera
- AS09 461a
- 美国
- 2025年07月13日
- western blot (WB)
- 兔
- 植物
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
兔抗拟南芥光系统I PsaD亚基多克隆抗体
- 抗体英文名：
PsaD|PSI-D subunit of photosystem I
- 浓度：
见说明书
- 应用范围：
western blot (WB)
- 宿主：
兔
- 适应物种：
植物
- 标记物：
无
- 克隆性：
多抗/单行
- 保存条件：
低温
- 亚型：
IgG
- 规格：
200 µl
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PsaD|PSI-D subunit of photosystem I介绍：
货 号：AS09 461
中文名称：兔抗拟南芥光系统I PsaD亚基多克隆抗体
英文名称：PsaD|PSI-D subunit of photosystem I
应用：western blot (WB)
规格：200 µl
价格：5175元
PsaD|PSI-D subunit of photosystem I简介：
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| APPLICATION INFORMATION | ||
| recommended dilution | 1 : 1000 with SuperSignal West Pico detection (WB) |
|
| expected | apparent MW | 17.9 | 20 (for Arabidopsis thaliana) |
|
| confirmed reactivity | Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Lactuca sativa, Oryza sativa, Physcomitrella patens, Spinacia oleracea, Synechocystis PCC 6803, Zea mays |
|
| predicted reactivity | plants (monocots, dicots and conifers), Bigelowiella natans, green algae |
|
| not reactive in | Synechococcus elongatus sp. PCC 7942 |
|
| additional information | this antibody is a replacement for former product, anti-PsaD AS04 046 |
|
| selected references | Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113 Fujii et al. (2015). Photoprotection vs Photoinhibition of Photosystem II in Transplastomic Lettuce (Lactuca sativa) Dominantly Accumulating Astaxanthin. Plant Cell Physiol. 2015 Dec 7. pii: pcv187. Daddy et al. (2015). A novel high light-inducible carotenoid-binding protein complex in the thylakoid membranes of Synechocystis PCC 6803. Sci Rep. 2015 Mar 30;5:9480. doi: 10.1038/srep09480. Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12. Cheng and He (2014). PfsR Is a Key Regulator of Iron Homeostasis in Synechocystis PCC 6803. PLoS One. 2014 Jul 10;9(7):e101743. doi: 10.1371/journal.pone.0101743. eCollection 2014. Tomizioli et al. (2014). Deciphering thylakoid sub-compartments using a mass spectrometry-based approach. Mol Cell Proteomics. 2014 May 28. pii: mcp.M114.040923. |
|
| application example 10 µg of total leaf protein extracted with PEB (AS08 300) from (1) Zea mays, (2)Chlamydomonas reinhardtii, and (3) Spinacia oleracea were separated on 4-12%NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to nitrocellulose. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed withanti-PsaD (AS09 461, 1:1000, 1h) and secondary anti-rabbit (1:40000, 1h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with SuperSignal West Pico(Thermo Scientific) using a GenoPlex Chemi CCD (accumulated signal 10 x 30s exposure, bin 2x2). |
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文献和实验Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach
Methods to prepare photosystem (PS)I reaction center (RC) subunit polypeptides are described. For PsaC, PsaD, PsaE, and PsaY, the filtrate resulting from the ultrafiltration in Chapter 6 is used for preparative sodium dodecyl sulfate
Isolation of Photosystem I Particles From Spinach
. The particles consist of PsaA, PsaB, PsaC, PsaD, PsaE, and PsaY. Assay methods that involve SDS-PAGE and P700 determination are also presented.
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