- ￥5175
- Agrisera
- AS09 461a
- 美国
- 2025年07月13日
- western blot (WB)
- 兔
- 植物
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- 详细信息
- 技术资料
- 抗体名：
兔抗拟南芥光系统I PsaD亚基多克隆抗体
- 抗体英文名：
PsaD|PSI-D subunit of photosystem I
- 浓度：
见说明书
- 应用范围：
western blot (WB)
- 宿主：
兔
- 适应物种：
植物
- 标记物：
无
- 克隆性：
多抗/单行
- 保存条件：
低温
- 亚型：
IgG
- 规格：
200 µl
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PsaD|PSI-D subunit of photosystem I介绍：
货 号：AS09 461
中文名称：兔抗拟南芥光系统I PsaD亚基多克隆抗体
英文名称：PsaD|PSI-D subunit of photosystem I
应用：western blot (WB)
规格：200 µl
价格：5175元
PsaD|PSI-D subunit of photosystem I简介：
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| APPLICATION INFORMATION | ||
| recommended dilution | 1 : 1000 with SuperSignal West Pico detection (WB) |
|
| expected | apparent MW | 17.9 | 20 (for Arabidopsis thaliana) |
|
| confirmed reactivity | Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Lactuca sativa, Oryza sativa, Physcomitrella patens, Spinacia oleracea, Synechocystis PCC 6803, Zea mays |
|
| predicted reactivity | plants (monocots, dicots and conifers), Bigelowiella natans, green algae |
|
| not reactive in | Synechococcus elongatus sp. PCC 7942 |
|
| additional information | this antibody is a replacement for former product, anti-PsaD AS04 046 |
|
| selected references | Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113 Fujii et al. (2015). Photoprotection vs Photoinhibition of Photosystem II in Transplastomic Lettuce (Lactuca sativa) Dominantly Accumulating Astaxanthin. Plant Cell Physiol. 2015 Dec 7. pii: pcv187. Daddy et al. (2015). A novel high light-inducible carotenoid-binding protein complex in the thylakoid membranes of Synechocystis PCC 6803. Sci Rep. 2015 Mar 30;5:9480. doi: 10.1038/srep09480. Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12. Cheng and He (2014). PfsR Is a Key Regulator of Iron Homeostasis in Synechocystis PCC 6803. PLoS One. 2014 Jul 10;9(7):e101743. doi: 10.1371/journal.pone.0101743. eCollection 2014. Tomizioli et al. (2014). Deciphering thylakoid sub-compartments using a mass spectrometry-based approach. Mol Cell Proteomics. 2014 May 28. pii: mcp.M114.040923. |
|
| application example 10 µg of total leaf protein extracted with PEB (AS08 300) from (1) Zea mays, (2)Chlamydomonas reinhardtii, and (3) Spinacia oleracea were separated on 4-12%NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to nitrocellulose. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed withanti-PsaD (AS09 461, 1:1000, 1h) and secondary anti-rabbit (1:40000, 1h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with SuperSignal West Pico(Thermo Scientific) using a GenoPlex Chemi CCD (accumulated signal 10 x 30s exposure, bin 2x2). |
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