- ￥6600
- Agrisera
- AS09 489a
- 美国
- 2025年07月16日
- ELISA (ELISA), western blot (WB)
- 兔
- 植物
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- 详细信息
- 技术资料
- 抗体名：
兔抗拟南芥质膜水孔蛋白PIP1;1, PIP1;2, PIP1;3 多克隆抗体
- 抗体英文名：
PIP1;1,PIP1;2,PIP1;3|aquaporins
- 浓度：
见说明书
- 应用范围：
ELISA (ELISA), western blot (WB)
- 宿主：
兔
- 适应物种：
植物
- 标记物：
无
- 克隆性：
多抗/单行
- 保存条件：
低温
- 亚型：
IgG
- 规格：
100 µl
供应商：上海经科化学科技有限公司
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QQ:472482400(上海经科）
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PIP1;1,PIP1;2,PIP1;3|aquaporins介绍：
货 号：AS09 489
中文名称：兔抗拟南芥质膜水孔蛋白PIP1;1, PIP1;2, PIP1;3 多克隆抗体
英文名称：PIP1;1,PIP1;2,PIP1;3|aquaporins
应用：ELISA (ELISA), western blot (WB)
规格：100 µl
价格：6600元
PIP1;1,PIP1;2,PIP1;3|aquaporins简介：
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| APPLICATION INFORMATION | ||
| recommended dilution | 1: 8000 (ELISA), 1: 1000 with standard ECL (WB) |
|
| expected | apparent MW | 30.66 | 28 kDa (Raphanus sativus) |
|
| confirmed reactivity | Arabidopsis thaliana, Nicotiana tabacum, Raphanus sativus, Zea mays |
|
| predicted reactivity | dicots including: Lupinus sp. , Solanu lycopersicum, Ricinus communis, monocots including:Hordeum vulgare, Oryza sativa, trees: Populus trichocarpa, |
|
| not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
|
| additional information | Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC. Manufactured by Operon Biotechnologies. |
|
| selected references | Pengelly et al. (2014). Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts. J Exp. Botany, doi:10.1093/jxb/eru156. |
|
| application example
1 µg and 10 µg of crude membrane fraction/lane fromArabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP1s ntibodies (diluted in 1: 500 (left panel) and 1: 1000 (right panel), AS09 489, and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. |
 |
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