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兔抗谷胱甘肽还原酶多克隆抗体
GR|glutathione reductase
见说明书
western blot (WB), immunolocalization (IL), immuno
兔
植物
无
多抗/单行
低温
IgG
100 µl
供应商:上海经科化学科技有限公司
服务热线:400-0199-638
QQ:472482400(上海经科)
微信号:shjkchem
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GR|glutathione reductase介绍:
货 号:AS06 181
中文名称:兔抗谷胱甘肽还原酶多克隆抗体
英文名称:GR|glutathione reductase
应用:western blot (WB), immunolocalization (IL), immuno
规格:100 µl
价格:5250元
GR|glutathione reductase简介:
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APPLICATION INFORMATION | ||
recommended dilution | 1: 5000 with standard ECL (WB), needs to be optimized, 2 µg (IP), 1: 1000 (IL) |
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expected | apparent MW | 54 kDa |
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confirmed reactivity | Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Pisum sativum, Silene vulgaris, Solanum tuberosum, Zea mays, Scenedesmus quadricauda (algae) |
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predicted reactivity | dicots including: Brassica rapa, Glycine max; |
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not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
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additional information | This antibody will recognize the chloroplastic and cytoplasmic forms of the enzyme. |
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selected references | Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1–12. Shaw et al. (2015). β-aminobutyric acid mediated drought stress alleviation in maize (Zea mays L.). Environ Sci Pollut Res Int. 2015 Sep 29. Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4. Kovácik et al. (2013). Oxidative stress, uptake and bioconversion of 5-fluorouracil in algae. Chemosphere. 2013 Dec 28. pii: S0045-6535(13)01679-2. doi: 10.1016/j.chemosphere.2013.11.074. (immunolocalization in algae) Sobrino-Plata et al. (2013). Specific stress responses to cadmium, arsenic and mercury appear in the metallophyte Silene vulgaris when grown hydroponically. RSC Advances, Jan 24. |
application example 10 µg of total protein from (1) Arabidopsis thaliana leaf extracted with ProteinExtration Buffer, PEB (AS08 300), (2) Nicotiana tabaccum leaf extracted with PEB, (3)Zea mays extracted with PEB, (4) Hordeum vulgare leaf extracted with PEB, (5)Physcomitrella patens total cell extracted with PEB, (6) Chlamydomonas reinhardtiitotal cell extracted with PEB, (7) Synochocystis elongatus total cell extracted with PEB, extracted with PEB, were separated on 4-12% NuPage (Invitrogen) LDS-PAGEand blotted 1h to nitrocellulose. Blots were blocked in 2 % low fat dry milk in TBS-T (0.1 % Tween 20) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:30 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 30 seconds with WEST PICO reagent according the manufacturers instructions. |
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