AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana AKT1Q38998 , At2g26650
host
rabbit
clonality
polyclonal
purity
affinity purified serum
format
lyophilized
quantity
200 µg
reconstitution
For reconstitution add 100 µl of sterile water.
storage
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
for detection images please, refer to the publication below
Antibody detects native and recombinant AKT1.
APPLICATION INFORMATION
recommended dilution
1: 50 with 125I (WB)
expected | apparent MW
96.9 kDa
confirmed reactivity
Arabidopsis thaliana
predicted reactivity
Arabidopsis thaliana
not reactive in
no confirmed exceptions from predicted reactivity known in the moment
additional information
In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
selected references
Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047. Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.