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AKT1

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  • ¥5565
  • Agrisera
  • AS09 516a
  • 美国
  • 2025年07月24日
  • western blot (WB)
  • 植物
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      兔抗拟南芥钾离子通道AKT1多克隆抗体

    • 抗体英文名

      AKT1|potassium channel AKT1

    • 浓度

      见说明书

    • 应用范围

      western blot (WB)

    • 宿主

    • 适应物种

      植物

    • 标记物

    • 克隆性

      多抗/单行

    • 保存条件

      低温

    • 亚型

      IgG

    • 规格

      200 µg

    供应商:上海经科化学科技有限公司

    服务热线:400-0199-638


    QQ:472482400(上海经科)


    微信号:shjkchem

    活动:消费积分可换充值卡!

    AKT1|potassium channel AKT1介绍:

    货 号:AS09 516

    中文名称:兔抗拟南芥钾离子通道AKT1多克隆抗体

    英文名称:AKT1|potassium channel AKT1

    应用:western blot (WB)

    规格:200 µg

    价格:5565元

    AKT1|potassium channel AKT1简介:

    PRODUCT INFORMATION
    background

    AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.

    immunogen

    KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650

    host

    rabbit

    clonality

    polyclonal

    purity

    affinity purified serum

    format

    lyophilized

    quantity 200 µg
    reconstitution For reconstitution add 100 µl of sterile water.
    storage

    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    tested applications

    western blot (WB)

    related products

    AS09 515 | anti-KC1 | potassium channel KAT3

    collection of antibodies to membrane transport system

    additional information

    for detection images please, refer to the publication below

    Antibody detects native and recombinant AKT1.

    APPLICATION INFORMATION
    recommended dilution

    1: 50 with 125I (WB)

    expected | apparent MW

    96.9 kDa

    confirmed reactivity

    Arabidopsis thaliana

    predicted reactivity

    Arabidopsis thaliana

    not reactive in

    no confirmed exceptions from predicted reactivity known in the moment

    additional information

    In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).

    selected references Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047.
    Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.

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    图标文献和实验
    相关实验
    • 【求助】求助:AKT1的基因序列

      jixianxiu1982 各位大侠,有谁知道AKT1的基因序列呀,我找了好久没找到。 doctormy 人类AKT1序列: http://www.ncbi.nlm.nih.gov/nuccore/33875493 jixianxiu1982 谢谢doctormy大侠 jixianxiu1982 我还想继续知道其中PH结构域的基因序列,该怎么找

    • Akt信号通路总况

      相关专题 Akt1, also known as "Akt" or protein kinase B (PKB). Its signalling pathway is as follows: 1. Binds to phospholipids in the membrane 2. Phosphorylation by PDK1 and PDK2 3. Lipid phosphatases

    • DExD⁄H框RNA解旋酶负调节拟南芥对低K+的忍耐

                土壤的营养对植物的生长和代谢过程非常重要, 植物需要从土壤中获取营养, 并且演化出在不同的营养条件下确保能够继续吸收营养的适应机制。 K+ 是重要的营养物质,在低K+ 胁迫下, 很多植物表现出了不同程度的症状,如叶片发黄、生长受抑制等。 过去的研究发现AKT1, HKT,KT⁄KUP⁄HAK家族的基因在K+ 转运中起到重要作 用,但是关于细胞如何感受不同浓度的K+ 知道的不多。         

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