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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
兔抗拟南芥钾离子通道AKT1多克隆抗体
- 抗体英文名:
AKT1|potassium channel AKT1
- 浓度:
见说明书
- 应用范围:
western blot (WB)
- 宿主:
兔
- 适应物种:
植物
- 标记物:
无
- 克隆性:
多抗/单行
- 保存条件:
低温
- 亚型:
IgG
- 规格:
200 µg
供应商:上海经科化学科技有限公司
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AKT1|potassium channel AKT1介绍:
货 号:AS09 516
中文名称:兔抗拟南芥钾离子通道AKT1多克隆抗体
英文名称:AKT1|potassium channel AKT1
应用:western blot (WB)
规格:200 µg
价格:5565元
AKT1|potassium channel AKT1简介:
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| APPLICATION INFORMATION | ||
| recommended dilution | 1: 50 with 125I (WB) |
|
| expected | apparent MW | 96.9 kDa |
|
| confirmed reactivity | Arabidopsis thaliana |
|
| predicted reactivity | Arabidopsis thaliana |
|
| not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
|
| additional information | In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell). |
|
| selected references | Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047. Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877. |
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文献和实验jixianxiu1982 各位大侠,有谁知道AKT1的基因序列呀,我找了好久没找到。 doctormy 人类AKT1序列: http://www.ncbi.nlm.nih.gov/nuccore/33875493 jixianxiu1982 谢谢doctormy大侠 jixianxiu1982 我还想继续知道其中PH结构域的基因序列,该怎么找
相关专题 Akt1, also known as "Akt" or protein kinase B (PKB). Its signalling pathway is as follows: 1. Binds to phospholipids in the membrane 2. Phosphorylation by PDK1 and PDK2 3. Lipid phosphatases
土壤的营养对植物的生长和代谢过程非常重要, 植物需要从土壤中获取营养, 并且演化出在不同的营养条件下确保能够继续吸收营养的适应机制。 K+ 是重要的营养物质,在低K+ 胁迫下, 很多植物表现出了不同程度的症状,如叶片发黄、生长受抑制等。 过去的研究发现AKT1, HKT,KT⁄KUP⁄HAK家族的基因在K+ 转运中起到重要作 用,但是关于细胞如何感受不同浓度的K+ 知道的不多。
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