Dsp抗体

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Dsp|death-specific protein介绍：

货 号：AS10 935

中文名称：兔抗双子叶植物死亡特异蛋白多克隆抗体

英文名称：Dsp|death-specific protein

应用：western blot (WB)

规格：200 µl

价格：5085元

Dsp|death-specific protein简介：

PRODUCT INFORMATION immunogen KLH-conjugated synthetic peptide derived from known sequences of DSP in diatoms, conserved in Dsp1 and Dsp2, UniProt: B6RD07 of Thalassiosira pseudonana and Skeletonema costatum and Dsp1 in Fragilariopsis cylindrus and Phaeodactylum tricornutum host rabbit clonality polyclonal purity serum format lyophilized quantity 200 µl reconstitution For reconstitution add 200 µl of sterile water. storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications western blot (WB)

APPLICATION INFORMATION
recommended dilution		1 : 10 000 with standard ECL (WB)
expected | apparent MW		25 | 25 kDa
confirmed reactivity		Emiliania huxleyi, Aureococcus anophagefferens, Fragilariopsis cylindrus, Phaeodactylum tricornutum, Skeletonema costatum, Thalassiosira pseudonana
not reactive in		Aureococcus anophagefferens
selected references		Thamatrakoln et al. (2013). Death-specific protein in a marine diatom regulates photosynthetic responses to iron and light availability. PNAS 2013 Dec 10;110(50):20123-8. doi: 10.1073/pnas.1304727110. Epub 2013 Nov 25.

application example



5 µg of total protein, extracted with LDS sample buffer (PSB) were separated on 4-20% SDS-PAGE and transferred to PVDF. Blot was blocked with 2% ECL block in TBS-T (0.1% Tween-20) for 1h at room temperature (RT) with agitation. Blot was incubated in primary antibody at a dilution of 1: 10,000 for 1h at RT with agitation. Antibody solution was decanted and the blot rinsed briefly twice with TBS-T, then washed once for 15 min and 3 times for 5 min. at RT with agitation. Blot was incubated in secondary antibody (horse radish peroxidase conjugated anti-rabbit IgG) at a dilution of 1:20 000, for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Image (20 sec. exposure) captured with a BioRad Chemidoc MP.
Predicted activity and molecular weights (Da) - Thalassiosira pseudonana Dsp1 (24572 Da) Dsp2 (25406 Da), Skeletonema costatumDsp1 (24848 Da) Dsp2 (26566 Da), Phaeodactylum tricornutum Dsp1 (26585 Da, Fragilariopsis cylindrus Dsp1 (20312 Da).

Competition assay (right half of blot): Same protein loads as for lanes 1-7. Antisera were pre-incubated for 90 min. in TBS-T with of an excess of the free peptide antigen ​