LhcSR1

供应商：上海经科化学科技有限公司

服务热线：400-0199-638

QQ:472482400(上海经科）

微信号：shjkchem

活动：消费积分可换充值卡！

LhcSR1介绍：

货 号：AS14 2819

中文名称：兔抗莱茵衣藻LHCSR1多克隆抗体

英文名称：LhcSR1

应用：West blot

规格：50 µl

价格：3975元

LhcSR1简介：

PRODUCT INFORMATION background LHCSR1 (Stress-related chlorophyll a/b binding protein 1) plays a role in an efficient enery dissipation process, called non-photochemical quenching (NPQ), in Chlamydomonas reinhardtii. immunogen KLH-conjugated synthetic peptide derived from LHCSR1 protein sequence from Chlamydomonas reinhardtii, UniProt: P93664 host rabbit clonality polyclonal, purity affinity purified serum in PBS pH 7.4 format lyophilized quantity 50 µl reconstitution For reconstitution add 50 µl of sterile water storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications western blot (WB) related products AS14 2766 | anti-LHCSR3, rabbit anitbodies AS15 3081 | anti-LhcSR (Physcomitrella patens), rabbit antibodies LHC available antibodies against pigment-binding proteins Collection of antibodies to Chlamydomonas proteins additional information to be added when available

APPLICATION INFORMATION
recommended dilution		1: 1000 with standard ECL (WB)
expected | apparent MW		27 kDa
confirmed reactivity		Chlamydomonas reinhardtii
predicted reactivity		Chlamydomonas reinhardtii
not reactive in		Phaeodactylum tricornutum
selected references		to be added when available, antibody released in July 2015

application example



10 ug of a total cell extract of Chlamydomonas reinhardtii: WT _P _HL_High CO2 (1), WT_P_HL (2), Npq4_P _HL (3), were separated on Bolt® 4-12% Bis-Tris Plus Gels (precast) and blotted to PVDF using Bolt® transfer system for 1h . Blots were blocked with 5% BSA/milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and
3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:10 000 in for 1h at RT with
agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.

P: photoautotrophically grown cells
HL: High light illumination(light intensity:500 μE)

Courtesy Dr. Roberta Croce, Biophysics of Photosynthesis Dep. Physics and Astronomy Faculty of Sciences VU University Amsterdam, The Netherlands