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- http://lucigen.com/store/docs/manuals/MA053-Phage-Display.pdf
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- 2026年04月09日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-80º C
- 英文名：
ER2738 Electrocompetent Cells
- 库存：
大量
- 供应商：
中北林格
- 规格：
12 rxns (DUOs)
Phage Display Competent Cells
产品说明书
- Perfect for antibody phage display or peptide phage display library creation
- The highest efficiency TG1 competent cells available: ≥4 × 1010 cfu/µg.
- Your only source for electrocompetent SS320 and ER2738 cells
- Amber suppressor and non-amber suppressor lines for maximum flexibility
- High efficiency MC1061 F- cells for cloning or control reactions
- Create larger libraries; speed discovery
- Bulk custom dispensing available with all of our phage display cells
MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage. This strain not included in the sample kit.
- TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression.
- SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.
- ER2738 Electrocompetent Cells. An amber suppressor strain (glnV) prepared as highly efficient (≥2 × 1010 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.™ Phage Display Kits.
 |
| Figure 1. Transformation efficiency of Lucigen's electrocompetent bacterial cells for phage display compared to competitor's specification. |
Table 1. Competent Cells for Phage Display libraries
| Cell Lines |
Transformation Efficiency |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
| TG1 Electrocompetent |
>4 × 1010 |
YES |
NO |
YES |
| SS320 (MC1061 F') Electrocompetent |
>4× 1010 |
YES |
NO |
YES |
| MC1061 F- Electrocompetent |
>4× 1010 |
YES |
NO |
YES |
| ER2738 Electrocompetent |
>2 × 1010 |
YES |
NO |
YES |
Custom Competent Cells
Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing!See Custom Competent Cells for detailed information.
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Ordering
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Specifications
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Manuals
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Resources
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells.
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文献和实验Belcher/Knight: Electrocompetent Cells
Electrocompetent Cells From Danijela Dukovski at Harvard Medical School. This protocol works well. --Julie Norville Materials DI water 10% Glycerol Special Equipment Centrifuge Ice water bath Liquid
Transformation of Electrocompetent E. coli with Blue/White selection
to determine the efficiency of competent cells: Efficiency (Transformants/µg) = colonies/plate x (Bacterial dilution factor) x 20,000 Highly competent cells should have ~50 colonies on the plate with a 1/10 dilution of the control DNA
Preparation of Electrocompetent Cells and Electroporation of Plasmid DNA
Preparation of Electrocompetent Cells and Electroporation of Plasmid DNA Inoculate 5-ml L-broth with a single colony of E. coli . Incubate 5 hours to overnight at 37°C on a roller or with moderate shaking.
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