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- Lucigen
- http://lucigen.com/store/docs/manuals/MA019_Ecloni%20EXPRESS_Chemically_Competent.pdf
- 60401-3
- 2026年01月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80º C
- 英文名：
E. cloni® EXPRESS BL21(DE3) Chemically Competent Cells
- 库存：
大量
- 供应商：
中北林格
- 规格：
48 rxns
E. cloni® EXPRESS BL21(DE3) Competent Cells
产品说明书
- Best value for routine protein expression... available as Electrocompetent and Chemically Competent Cells.
Lucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells (Figure 1), which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (> 5 × 109 cfu/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment (Figure 2).
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| Figure 1. Transformation efficiency comparison of commercially available BL21(DE3) competent cells.
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| Figure 2. Comparison of cloning and protein expression procedures. |
E. cloni EXPRESS BL21(DE3) strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.
NOTE: For best results in expressing proteins that may be toxic, we recommend using OverExpress Cells. E. cloniEXPRESS cells are also available as chemically competent cells. With a transformation efficiency of >1 × 107 cfu/µg and very economical prices, EXPRESS Chemically Competent Cells offer superior value for everyday protein expression work.
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Ordering
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Specifications
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Manuals
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Resources
Each E. cloni EXPRESS Kit contains: the indicated E. cloni EXPRESS Electrocompetent or Chemically Competent Cells in DUO packaging (2 transformations per tube), Expression Recovery Medium (lactose minus), pUC19 Positive Control Plasmid, and complete protocols.
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文献和实验TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
CCMB buffer to make chemical competent cell
, but you need a big clone or several clones. :D LB media is OK, OD less than 0.8 is OK This protocol has been tested on TOP10, MachI and BL21(DE3) , DH5α cells. 1 Preparing glassware and media 2 Preparing seed stocks 3 Preparing competent cells 4 CCMB80
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