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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 供应商:
普诺赛
- 英文名:
MEM(Richter's modification),不含酚红
- 规格:
500mL
细胞生长培养基是用以维持细胞生长增殖之用,含血清比例较大。生长培养基是培养细胞工作中最主要的培养用液,其组成为:基础培养基80%~90%,血清(多用胎牛血清) 10%~20%,抗生素(多用青霉素100U/mL和链霉素100μg/mL)。这是适用于一般细胞的培养基组成,为培养特定细胞,还要选择适应性培养基,可能尚需添加基本培养基中缺少或该细胞需要的成分。
细胞维持培养基是为维持细胞缓慢生长或不死的培养基,血清含量甚少。一般细胞因缺少生长因子不能增殖,生长缓慢;但发生转化或癌细胞因自身有产生促生长增殖因子的能力,虽在血清缺少情况下仍能生长;因此维持液可用于选择发生恶性转化的细胞,其组成为:基本培养基95%,血清2%~5%,抗生素(多用青霉素100U/mL和链霉素100μg/mL)。此处需注意,如培养正常细胞完全不加血清,可维持细胞不死,但时间过长细胞难免退化。
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文献和实验alkylation of protein that has been adsorbed onto a sequencing membrane is also presented. Reversible modification of cysteine residues by way of sulfitolysis is described, and a protocol for oxidation with performic acid for amino acid compositional analysis
The ability to alter the mouse genome through homologous recombination in their embryonic stem (ES) cells, and propagate the modification through their germ-line, has revolutionized biomedical research. Such gene-targeted mice have afforded
CAUTION: Sodium cyanoborohydride is a highly toxic compound. Follow all precautions recommended in the Material Safety Data Sheet. Do not attempt to sniff the compound's odor, and be aware that exposing it to strong acid will result
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