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- 2025年07月16日
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文献和实验. For example, novel DNA-binding proteins have been created by using phage display to alter the DNA-binding specificity of a zinc-finger protein (ZFP)2-7 . Although phage display is an effective method, it is labor-intensive and time-consuming
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
Studies of the Ubiquitin Proteasome System
of E3 Enzymes Expressed in In Vitro Translation Systems Alternate Protocol 2: Determination of Ubiquitin Chain Variant Phenotype Basic Protocol 7: Chelation of Zinc from RING‐ and PHD‐Finger E3s Alternate Protocol 3: Inhibition of HECT Domain E
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