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- 2025年07月15日
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文献和实验; stippled line, control staining without primary antibody. Screening for upregulation of target genes in human cell lines. We delivered millions of TFZF s into the human squamous carcinoma cell line A431 using a retroviral vector, pMX-IRES-GFP15 (Fig
supernatant by a sandwich ELISA capturing apoB with a polyclonal goat anti-human apoB antibody (Chemicon International). apoB detection was performed with a horseradish peroxidase-conjugated goat anti-human apoB-100 polyclonal antibody (Academy Bio-Medical
Intracellular Immunofluorescent Staining for Flow Cytometry
for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 detection needs at least 24 hours stimulation. In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines
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