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文献和实验Inducing RNAi with siRNA Cocktails Generated by RNase III
a number of long dsRNA substrates. Human GAPDH, La, and c-FOS dsRNA (200 bp) was prepared by in vitro transcription ( Silencer ™ siRNA Cocktail Kit (RNase III); See Materials and Methods). The dsRNA was digested using 1 U RNase III
Activation and Expansion of Human Treg Cells
from the magnet. （5）Resuspend the washed Dynabeads in the same volume of Buffer 1 as the initial volume of Dynabeads. 2. Isolation of Human Treg Cells For isolation of human Treg cells it is recommended to use the Dynal CD4 CD25 Treg Kit. 3. Expansion
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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