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- EKU06863
- 2025年07月07日
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Models of Inflammation: Carrageenan Air Pouch
) ELISA kit(s) specific for inflammatory mediators (e.g., TNF‐α, IL‐1β, IL‐6, IL‐10, prostaglandins; Thermo Scientific) Isotonic phosphate‐buffered saline (PBS
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