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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
实验试剂 1. CytoTune™ Sendai Reprogramming Vectors Note: For successful reprogramming, you need all four reprogramming vectors. 2. Human neonatal foreskin fibroblast cells to reprogram 3. Gibco® Mouse
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
3) Transfer cells to an RNase-free centrifuge tube and pellet by centrifugation at 300 x g for 5 min. (In our lab, use 1800rpm in the eppendorf centrifuge 5415D). Cell pellets can be stored at –70°C for later use or used directly in the procedure
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