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- 2025年07月14日
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
, immunostaining of the chromosomes with a protein of interest, and counterstaining with Giemsa and Hoechst. RELATED INFORMATION Technical modifications incorporated into this protocol that have enabled more reproducible patterns of banding
coronavirus, porcine hemagglutinating encephalomyelitis virus, equine coronavirus, bovine coronavirus (BCoV), and wild-ruminant coronaviruses. The presence of a hemagglutinin-esterase (HE) surface glycoprotein in addition to the viral spike protein
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
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