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文献和实验) will phosphorylate its membrane-bound substrates, which include pp60src (Src) and MARCKS (mynstoylated alanine-rich C kinase substrate). Fourth, membrane binding is required for the biological activity of proteins such as Src and Ras. All these proteins bind
Identification of a Mutant Kinase/ATP Analog Pair
to the epitope tag on the protein kinase of interest A phospho-specific antibody to a known substrate of your kinase (if available) Gels SDS-PAGE gel Special
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
phosphatase‐conjugated secondary antibody Substrate 104 (or other alkaline phosphatase substrate; Sigma‐Aldrich) ELISA substrate
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