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文献和实验Human IL-2 Enzyme Immunoassay (EIA)
-2 EIA is an in vitro enzyme-linked immunosorbent assay for quantitatively detecting human IL-2 in biological fluids (e.g. serum, plasma) and in cell culture supernatants. The kit consists of a 96 well microtiter plate that has been precoated with anti-IL
28 Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use
This chapter will describe a method used to produce human megakaryocytes in vitro (see Fig. 1 ), in high yield, for a clinical product to supplement progenitor cell transplants. Producing significant numbers of megakaryocytes from human
Immunity | 伤口愈合时为什么会痒?陈万军团队发现竟然和这个免疫细胞有关!
RNA 测序,作者筛选得到 IL-31 作为皮肤切口后诱发瘙痒的候选因子。qPCR 和 ELISA 检测显示 IL-31 表达在创面愈合过程中升高,并在切口后第 5 天达到高峰。在敲除了小鼠 IL-31 后,小鼠在皮肤愈合过程中搔抓的行为明显减少,表明 IL-31 是导致伤口愈合过程中的瘙痒反应的主要因素。图片来源:Immunity那么,IL-31 是如何引起瘙痒的呢?作者发现在切口后 5 天支配伤口部位的背根神经节感觉神经元在愈合过程中 Il31ra(IL-31 受体)、Trpv1(辣椒素受体
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