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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
Overview of the Quantitation of Protein Interactions
, T.A., and Ciardelli, T.L. 1996. Kinetic analysis of ligand binding to interleukin‐2 receptor complexes created on an optical biosensor surface. Protein Sci. 5:2468‐2478.
Overview of Protein Microarrays
1:189‐203. Chao, G., Lau, W.L., Hackel, B.J., Sazinsky, S.L., Lippow, S.M., and Wittrup, K.D. 2006. Isolating and engineering human antibodies using yeast surface display. Nat
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