Mouse Heat Shock 70kDa Protein

Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures

with chaperones in these cells is of interest. Furthermore, Pb and Cu bind strongly to the molecular chaperone heat shock 70 kDa protein Hspa5, also known as glucose-regulated protein 78 (Grp78) or immunoglobulin-binding protein (BiP). This chapter describes

siRNAs结合生物芯片的实验设计

should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example

Genomic DNA Extraction - PureLink

below. Set 2 water baths or heat blocks at 55°C and 70°C, respectively. Place ~25 mg of minced mammalian tissue into a sterile microcentrifuge tube. Add 180 µl Lysis Buffer (L6) and 20 µl Proteinase K (supplied with the kit) to the tube