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- Biomatik
- EKU04434
- 2025年07月14日
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文献和实验Studies of the Ubiquitin Proteasome System
Calf intestinal alkaline phosphatase (CIAP; Roche) QIAquick Gel Extraction Kit (Qiagen) T4 DNA ligase kit (Takara) TOP10 chemically competent E. coli strain DH5α (Invitrogen) or other suitable host
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
Methods to Detect Protein Glutathionylation
Backos, D.S., Fritz, K.S., Roede, J.R., Petersen, D.R., and Franklin, C.C. 2011. Posttranslational modification and regulation of glutamate‐cysteine ligase by the alpha,beta‐unsaturated aldehyde 4‐hydroxy
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