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- EKU04100
- 2025年07月08日
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文献和实验and ordered, (2) the oligonucleotides are used as primers in one or more PCRs with the promoter-containing template included in the kit, and (3) the resulting PCR product is column purified. Ambion scientists typically use a loop sequence of 5'-UUUGUGUAG
must be prepared. Web-based tools for designing these templates are available for the following Ambion kits/products: Silencer siRNA Construction Kit [ Product Info ] [ Design Tool ] pSilencer siRNA Expression Vectors [ Product Info ] [
such as Stbl3 (Life Technologies,Carlsbad, CA, USA) or other recombination-defi cient strain isrecommended to avoid recombination of repetitive elementsin the plasmids. 12. Luria Broth (LB) media. 13. Ampicillin. 14. Spin miniprep kit. 15. Plasmid
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