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文献和实验) (4) , to the final purified protein. In addition, protocolsto assay Wnt activity are described. Two key observations have made the purification of Wntspossible: 1) inclusion of detergent to facilitate solubility of thehighly hydrophobic Wnt protein
A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay
in transglycosylation reaction. In the assay, UDP was coupled to the conversion of phosphoenolpyruvate to pyruvate using pyruvate kinase. Using a commercial pyruvate assay kit, the pyruvate was converted to a red terminal product, which could be photometrically measured
licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit（cat NO:EKS-420A）?可否说一下应用的经验？注意事项等？还有，该试剂盒的说明书有几处地方不是很懂，请大家帮忙看一下： 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
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