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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
常温
- 英文名:
Percoll
- 库存:
现货
- CAS号:
65455-52-9
- 规格:
250ml/1L/100ml/6x1L/25ml
| 规格: | 250ml | 产品价格: | ¥1938.0 |
|---|---|---|---|
| 规格: | 1L | 产品价格: | ¥3672.0 |
| 规格: | 100ml | 产品价格: | ¥379.0 |
| 规格: | 6x1L | 产品价格: | ¥21705.0 |
| 规格: | 25ml | 产品价格: | ¥263.0 |
Cytiva/思拓凡(原GE Healthcare) 17089101 Percoll 1L 现货
Cytiva/思拓凡(原GE Healthcare) 17089102 Percoll 250ml 现货
Cytiva/思拓凡(原GE Healthcare) 17089103 Percoll 100 ml 现货
Cytiva/思拓凡(原GE Healthcare) 17089105 Percoll 25ml 现货
Cytiva/思拓凡(原GE Healthcare) 17089109 Percoll 6x1L 现货
概述:
Percoll是一种包有polyvinylpyrrolidone (PVP)的硅胶颗粒的水溶液。渗透压很低(<20mosm/kg H2O), 粘度也很小,可形成高达1.3g/ml密度。由于Percoll扩散系数低,所以形成的梯度十分稳定。此外,Percoll不穿透生物膜,对细胞无毒害,因此广泛用于分离细胞、亚细胞成分、细菌及病毒,还可将受损细胞及其碎片与完好的活细胞分离。
参数:
Composition:Silica sol with non-dialysable PVP coating
Density (g/ml):1.130 ±0.005
Conductivity (mS/m):max. 100
Osmolality (mOsm/kg H2O):max. 25
Viscosity (cP):max 15
pH:9.0 ±0.5
储存条件:
Percoll PLUS/Percoll are supplied sterile and may be stored unopened for up to 5 years at room temperature. When opened, it should be stored at 4°C to 8°C.
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文献和实验JA Pille, ML Riess. (2021). Potential Effects of Poloxamer 188 on Rat Isolated Brain Mitochondria after Oxidative Stress In Vivo and In Vitro. Brain Sciences (2076-3425)
Daher, C., Vimeux, L., Stoeva, R., Peranzoni, E., Bismuth, G., Wieduwild, E., … Feuillet, V. (2019). Blockade of β-adrenergic receptors improves CD8+ T-cell priming and cancer vaccine efficacy. Cancer Immunology Research, canimm.0833.2018. doi:10.1158/2326-6066.cir-18-0833
Huang, B., Liu, R., Wang, P., Yuan, Z., Yang, J., Xiong, H., … Li, L. (2020). CD8+CD57+ T cells exhibit distinct features in human non-small cell lung cancer. Journal for ImmunoTherapy of Cancer, 8(1), e000639. doi:10.1136/jitc-2020-000639
Percoll是一种包有乙烯吡咯烷酮的硅胶颗粒。渗透压很低( 不同浓度(密度)Percoll溶液的制备: 先用9份Percoll与1份8.5% NaCl或1.5MPBS混合达到生理性渗透压,然后用生理溶液(0.85% NaCl或0.15M PBS)稀释到所需浓度。 Percoll浓度(%) 70 60 50 40 30 20 比重g/ml
Equilibrium Density Gradient - Percoll
Materials Ultracentrifuge and swinging bucket rotor Percoll NaCl solutions with the following osmolarities:200, 300 and 400 milliosmols Whole blood Hemacytometer
Percoll不连续密度梯度沉淀法分离纯化淋巴细胞和淋巴母细胞 撰写 金伯泉 -------------------------------------------------------------------------------- (一) 原理 Percoll











