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文献和实验28 CTS™ per 5 x 108 CD3 T cells and immediately proceed to the next step. 5) Place the bag on a cell mixer and mix for 30 min at room temperature to gently mix the cells and the Dynabeads®. [If the starting sample contains less than 25% CD
RNase and DEPC Treatment: Fact or Laboratory Myth
. Figure 1. Effect of Autoclaving on RNase Activity. Various concentrations of RNase A were added to PBS and autoclaved for 25 minutes. 1 µl of each solution was mixed with 1 ng of a 5 x 104 cpm RNA probe which was 304 bases long and incubated
相关专题 总有一种转染方法适合你 近日,罗氏公司新推出了两款高性能的DNA转染试剂,为其细胞分析产品线再添新军。这两款名为X-tremeGENE 9和X-tremeGENE HP的转染试剂沿用了X-tremeGENE siRNA 转染试剂的前缀,应该与大名鼎鼎的Fugene 6不属于一类。据罗氏介绍,这两个产品是多组分的独特试剂,并非脂质体。它们能够提供高的转染效率、重复性和细胞存活率。 这两款新产品都融合了罗氏二十
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