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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询说明书
- 库存:
10
- 供应商:
上海乔羽生物科技有限公司
- 规格:
250 µl (25 µmol)
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文献和实验. Transfer 5 µl of the PCR product to another PCR tube. Bring the volume to 25 µl with a newly prepared PCR mix containing [{alpha}-32P]-dCTP (10 µCi/µl, specific activity 3000 Ci/mmol) and fresh dNTPs. The amount of [{alpha}-32P]dCTP
Random primer generation of [32P]-labelled DNA probes
DNA probes are prepared using a modification of the method of Feinberg and Vogelstein, (1983). You will need: Nuclease-free BSA [a-32P]-dCTP (Amersham or DuPont) Klenow DNA Polymerase (New England Biolabs or Pharmacia) 500mM EDTA
DNA probes are prepared using a modification of the method of Feinberg and Vogelstein
DNA probes are prepared using a modification of the method of Feinberg and Vogelstein, (1983). You will need: Nuclease-free BSA [a-32P]-dCTP (Amersham or DuPont) Klenow DNA Polymerase (New England Biolabs or Pharmacia) 500mM EDTA
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