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- Roche
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- 2025年12月20日
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详询说明书
- 库存：
10
- 供应商：
上海远慕生物
- 规格：
5 ml
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文献和实验Clonality - X Chromosome Inactivation Assay
is utilized for analysis of archival samples, we highly recommend that replicate experiments (multiple independent dissections, triplicate PCR reactions, etc.) be used to verify results. A: LCM and Proteinase K Treatment Obtain microdissected cells using
A safe method of extracting DNA from Coccidioides immitis
with 10 ul of 10 mg/ml Proteinase K for 2-3h, and then incubated again at 80 C for 10 min. The DNA is then extracted with chloroform- phenol and precipitated with isopropanol, following Lee and Taylor (In: M. A. Innis et al, eds. PCR Protocols, pp. 282-287
. Therefore, when this technique is used to analyze archival samples, it is highly recommended that replicate experiments (multiple independent dissections, triplicate PCR reactions, etc.) be used to verify results.A: LCM and Proteinase K TreatmentObtain microdissected cells using
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