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- 2025年12月15日
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上海远慕生物
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文献和实验well as siRNAs that are expressed in vivo. The only exception is that siRNA sequences to be expressed in vivo should not contain a run of 4 or 5 A's or T's, as these can act as termination sites for Polymerase III. For traditional cloning into pSilencer vectors
to be expressed in vivo should not contain a run of 4 or 5 A's or T's, as these can act as termination sites for Polymerase III. For traditional cloning into pSilencer vectors, two DNA oligonucleotides that encode the chosen siRNA sequence are designed
Cassette and Its Relationship to the RNA Target Sequence. Ambion's Recommended Procedure for siRNA Hairpin Design The following recommendations for siRNA hairpin design and cloning strategy are made based on research by Ambion scientists
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