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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Hylauronic acid, Cy5.5 labeled
- 供应商：
默瑞生物
- 规格：
5 mg
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文献和实验Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
an alternate approach, the Nucleic Acid Programmable Protein Array (NAPPA) is constructed by spotting protein?encoding plasmid DNA at high density, in addressable fashion, on an array surface. Proteins are subsequently generated in situ
) and GST-FRB and (His)6-FKBP12 were produced recombinantly in Escherichia coli. IKBalpha and (His)6-FKBP12 were labeled with Cy3 and Cy5, respectively, using monofunctional reactive dye from Amersham Pharmacia Biotech (Newark, NJ) and following
for Cy5-dUTP is less than that of Cy3-dUTP, so more RNA is labeled to achieve more equivalent signal from each species. 2. Heat to 65°C for 10 minutes and cool on ice for 2 minutes. 3. Add 23 µl of reaction mixture containing either Cy5-dUTP
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