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文献和实验常用化学试剂分子量 Molecular Weights of Commonly Used Chemicals
Potassium Chloride 74.55 dATP, disodium salt 535.1 Potassium Phosphate , anhydrous, monobasic 136.1 Bis 154.17 Potassium Phosphate, trihydrate, dibasic
Human Placental Alkaline Phosphatase Stain
off staining solution with water. STOCK SOLUTIONS XPHOS - 100X solution is 10 mg/ml 5-bromo-4-chloro-3-indolyl phosphate, disodium salt (Sigma) in water, store at -20°C. NBT - 100X solution is 50 mg/ml Nitro Blue tetrazolium (Sigma) in 70
In Vitro Transcription Assay (Run-off Assay) using Permeabil
to give a final concentration of 10 μg/ml 7.6 μl of Creatine Phosphate to give a final concentration of 10 mM 20 μl of Combined NTPs to give a final concentration of 220 μM 25 μl of [32 P] GTP (>3,000 Ci/mmol) (CAUTION! see Hint #1) The total volume should be 304
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