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- 2025年07月08日
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文献和实验proteinogenic/free amino acids or central carbon metabolites, and (2) a mathematical model that relates the unknown fluxes to the measured isotopomers. Modeling requires a thorough knowledge of the structure of the underlying metabolic network, often available
SYBR Green Quantitative PCR Protocol
12μl of master mix for each well, plus some excess1 Rxn: 10μl SYBR Green Mix 52 Rxn: 520μl SYBR 0.4μl Forward Primer (10μM stock) 20.8μl F
The pararosanilin is dissolved in the distilled water and the hydrochloric acid is added. The solution is heated gently, cooled, filtered and stored in aliquots in a refrigerator. B) Sodium Nitrite Sodium nitrite 2g Distilled water 50
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