The Human RUNX1 Gene qBiomarker Somatic Mutation PCR Array allows the detection of 20 unique mutations in the human RUNX1 gene. RUNX1, a transcription factor, plays a role in normal hematopoiesis. Previous scientific reports have shown a strong association between mutations in the RUNX1 gene and acute myeloid leukemia. The RUNX1 mutations found in cancer samples are predominately missense point mutations, about half of which occur in its Runt domain, which is responsible for DNA-binding and protein-protein interactions. The DNA sequence mutation assays in the array detect the most frequent mutations in RUNX1 compiled from over 400 reported cancer sample sequences. Each mutation assay is bench-tested for the sensitive detection of a low percentage of mutation-containing DNA in a background of wild-type genomic DNA. The 96-well format of this array profiles the mutation status of 4 samples, while the 384-well array format profiles 16 samples, and each set of plates includes qPCR mastermix. Using real-time PCR, research studies can easily and reliably detect a panel of mutations in the RUNX1 gene with this array.