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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
41.iii) LiCl buffer for ChIP Lymphocyte separation medium (LSM) Lymphocytes, human leukapheresis pack from a blood bank The pack contains leukocytes enriched by apheresis from human peripheral blood. Micrococcal nuclease
, and the clear side of the case. Put the case in the holder, black side of the case facing black side of the holder. Run the transfer at 100V for 1 hour.3. Meanwhile, prepare 500 ml of AC Buffer (+ Tween):50 ml glycerol (= 10% glycerol)10 ml 5M NaCl (= 100mM NaCl
paper, 2nd Scotch Brite Pad, and the clear side of the case. Put the case in the holder, black side of the case facing black side of the holder. Run the transfer at 100V for 1 hour. 3. Meanwhile, prepare 500 mL of AC Buffer (+ Tween): 50 mL
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