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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
Eppendorf tube. The tube can be filled with powdered mycelium - Shake for 15-20 min (Eppendorf Rotationsmischer 3300) - Centrifuge for 10 min, 10,000 rpm - Transfer the upper phase into an equal volume of phenol (saturated with 0.1 M Tris HCl, pH
CRESYL FAST VIOLET TECHNIQUE FOR NISSL
SECTIONS 8µm paraffin wax sections SOLUTIONS STAINING SOLUTION Cresyl Fast Violet-1g Distilled water-100cm3 Acetic acid-0.25cm3 METHOD Take sections
An Ultra-fast method of DNA extraction from Neurospora
centrifuge at maximum speed for 1 minute. Add an additional 150 µl and repeat. Extraction Buffer 2M NaCl 0.4 Deoxycholic acid (sodium salt) Sigma D-6750 1.0 Brij 58 (polyoxyethylene 20 cetyl ether ) Sigma P-5884 DE/GTC 8.5 g of acid washed
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