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文献和实验nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA was constructed with a 5' biotin
Preparation of Nested Deletions
acetate sheet on bottom of original 150 mm plate and identify positive plaques. 13) Pick positive plaques by stabbing with a plastic transfer pipet, and squirt into 1 ml of SM containing a drop of chloroform. Let sit for 1-2 hrs at RT or overnight at 4℃
SSR GEL and Silver Staining Protocol
, grease or a bottom spacer. a. Place the larger plate onto caster so that it abuts the end wall of the caster. Moisten spacers with water and place them flush to the edge of glass against the caster wall. b. Place the top plate (notched or offset
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