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文献和实验nM 5'biotin end-labeled siRNA under gentle agitation, i.e. a rocking plate (Figure 1a). We utilized the EF1 alpha specific siRNA, EF52, shown previously to direct TGS in human cells (Morris et al. 2004). The EF52 siRNA was constructed with a 5' biotin
Preparation of Nested Deletions
acetate sheet on bottom of original 150 mm plate and identify positive plaques. 13) Pick positive plaques by stabbing with a plastic transfer pipet, and squirt into 1 ml of SM containing a drop of chloroform. Let sit for 1-2 hrs at RT or overnight at 4℃
Preparation of Nested Deletions
Preparation of Nested Deletions Procedure: 1) Need to cut 10 µg of plasmid in two spots ('A' and 'B') in polylinker. 'A'cut is 'ith a restriction enzyme which gives a 3' recessed end (exonuclease sensitive) next to insert
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