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文献和实验Plant DNA methylation analysis by bisulfite genomic sequencing
at sites outside of region intended for amplification to resolve structures like direct or inverted repeats that could interfere with PCR (see comment 2). To a 1.5ml reaction tube, Add genomic DNA dissolved in bidist. water (e.g. 2.5µg
_________________ 72C 10 minutes NOTE: The entire reaction can be used for a transformation without any further purification. MATERIALS 10X Taq Buffer: 0.5 M KCl 100 mM Tris-Cl, pH 8.5 1% Triton X-100 25 mM MgCl2 10 mM dNTP's pRS40X template DNA
Whole genome amplification protocol for ChIP-chip
of different factors (E2F family members, KAP1, CtBP2, ZNF217) as well as on histone modifications (H3me3K9, H3me3K27, H3me3K4) (Krig et al., 2007; O'Geen et al., 2007). Another benefit of the WGA amplification method is the ability to perform a second
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