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- 文献和实验
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上海希言科学仪器有限公司
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文献和实验at room temp for 5 min. 5. Add 4 ml of cold buffer P3. Cover with parafilm immediately and mix by gentle inversion 4-6 times. Incubate on ice 15 min. 6. Centrifuge in the F0650 rotor, using a blue rubber adapter sleeve, at 20,000 x g for 30 min
Qiagen MIDI protocol(QIAGEN plasmid Midi试剂盒)
by gentle inversion 4-6 times. Incubate on ice 15 min. 6. Centrifuge in the F0650 rotor, using a blue rubber adapter sleeve, at 20,000 x g for 30 min in the Beckman. Promptly pour supernatant into another round-bottom 13 ml polycarbonate tube
Generating and Analyzing Germ‐Free Mice
; outer cap nipples not shown in illustrations), and [16] disconnect the sterilization cylinder from transfer sleeve. Items not drawn to scale. NOTE: Outer and inner caps are secured using 18‐in rubber bands.
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