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文献和实验), CRFK(cat), NB324K(human), MCF7(human), WI38(human), and C636(mosquito) cells were effectively cryopreserved at −80�C while attached to the substratum of 25cm2 tissue culture flasks. This was accomplished using a serum free crypreservative recently
A novel method of growing fungi for DNA extraction
Preparation of fungi for DNA extraction typically involves growing cultures in liquid culture in Erlenmeyer flasks, Roux bottles or even microfuge tubes (Cenis 1992 Nucl. Acids Res. 20:2380). Growing fungal cultures in liquid may require
Add 0.3 ml of alcohol-orcinol reagent to each tube and mix well. Place the tubes in a boiling water bath for 20 minutes with marbles placed on top of each tube to prevent evaporation. Cool the tubes by immersion in an ice bath
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