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文献和实验定量RT-PCR (Quantitative RT-PCR)
The following procedure is a modified version of the NP-40 RNA extraction method for tissue culture cell lines (5). It is simple, fast and gives high yield of high quality RNA.
Protein extraction from whole tissues for IEF
Modified from that of Jay Thelen - University of Missouri-Columbia Phenol extraction followed by methanolic ammonium acetate precipitation - an effective protocol for sample preparation from protein-poor, recalcitrant tissues
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
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