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文献和实验Wageningen, Netherlands (Zabeau, 1992; Zabeau and Vos, 1993; Vos et al., 1995). The AFLP technology is covered by patents and patent applications owned by Keygene N.V. Both, Life Technologies (Gathersberg, MD, USA) and Perkin Elmer (Applied Biosystems
AFLP: not only for fingerprinting, but for positional cloning
only our "EcoRI-oligo.1" (adapter oligo with no extension) (see below), and our AFLP-PCR uses only 2-bp, 3'-extensions (see 2.2). Life Technology and Perkin Elmer protocols use the Vos et al. (1995) preamplification and AFLP-PCR. In our experience the single
AFLP: not only for fingerprinting, but for positional cloning
only our "EcoRI-oligo.1" (adapter oligo with no extension) (see below), and our AFLP-PCR uses only 2-bp, 3'-extensions (see 2.2). Life Technology and Perkin Elmer protocols use the Vos et al. (1995) preamplification and AFLP-PCR. In our experience the single
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