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文献和实验l with ddH2 O (take the volume of the nuclei into account).2. Incubate at 37℃ for 10 min.3. Lyse the nuclei by adding 1.5 ml of 6M Guanidium Chloride and shear the DNA by passing the sample through a 19 gauge needle on a syringe.4. Extract the lysate
Methods for DNA isolation(DNA分离方法)
syringe and a 25 gauge needle.(It may be helpful first to remove and discard the upper band). 13a.To remove the ethidium bromide,load the DNA sample onto an equilibrated 1.5 ml Dowex column,and collect 0.5 ml fractions.Equilibrate the Dowex AG resin
Genomic (Plant) DNA: Restriction Digestion for Sou
, potato and soybean use 10μg of total DNA. To avoid partial digests the DNA should be relatively clean. Performing the digestion in a very large volume (150μl) with an excess of enzyme also reduces partial digests because it dilutes salts
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