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文献和实验Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
Series of NaCl solutions (50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 700, 800, 900, and 1000 mM) in 1× PBS (store up to 1 month at 4°C) 50% (v/v
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
incubation Latex gloves Microscope, dissecting Microscope, phase-contrast Pencil with eraser end Rack for holding slides Razor blade Shaking platform Siliconized coverslips (Corning or equivalent; 22 x 22 mm)
In Situ HYBRIDIZATION TO TISSUE SECTIONS
1399 (poly-L), polylysine hydrobromide fraction 150,000-300,000]: Either/or combo of poly-D and poly-L lysine may be used to prepare a 50-100ug/ml solution in 10mM Tris-HCl, pH 8. Polymount (Polysciences, Inc.) Pronase (Calbiochem 537088
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