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上海希言科学仪器有限公司
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文献和实验and ligands that bind specific cell-associated molecules. Material Staining solution: L-15 medium (Sigma catalog L-99002) plus 2.5% FCS (4o C) Fluorescent-labeled antibodies 5 ml polystyrene tube 96 well V-bottom plate Propidium
2-D Polyacrylamide Gel Electrophoresis
Thiourea 2 g Chaps 0.5 g Mega 10 0.5 g OBG 250 µl Triton X-100 0.25 g Tris 0.4 g DTT 500 µl Pharmalytes or IPG buffer pH 3-10 (Amersham) 500 µl - mercaptoethanol 4.Add 10 µl tributylphosphine 2 mM,under the hood (2 mM final conc.) 5.Add
dryer (Life Technologies)Glass plates, 31.0 x 38.5 cm (Life Technologies)0.4 mm spacers (Life Technologies)Casting boot (Life Technologies)Shark tooth comb (Life Technologies)Small clampsWhatman blotting paper, 3 mm thicknessKodak Biomax MR or AR
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