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文献和实验on a rotary shaker. To drain reaction mixtures or washes, connect to house vacuum via a waste trap. The vessel should be fritted with a coarse frit. Siliconize the glass reaction vessel to avoid beads from sticking to the walls of the glass vessel. Prepare
Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
", abbreviated GLC. Compounds are injected onto the column and carried through it by the mobile phase; depending on their partition into the stationary phase, they move slower or faster. A sensitive detector is required at the end of the column to detect
Fluorescence Activated Cell Sorting (FACS)
. The laser light excites the dye which emits a color of light that is detected by the photomultiplier tube, or light detector. By collecting the information from the light (scatter and fluorescence) a computer can determine which cells are to be separated
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