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文献和实验An ELISA Lab-on-a-Chip (ELISA-LOC)
(ELISA-LOC) by integrating the washing step directly into an ELISA plate. LOM has been shown to be capable of creating complex 3D microfluidics through the assembly of a stack of polymer sheets with features generated by laser micromachining
Western Blotting and Immunostaining Protocol
core with the wedge. Set the power supply to deliver 30V and run for 1 hour. Larger proteins may require longer transfer times. At the end of the run, remove the blotting membrane from the sandwich assembly. Do not allow the membrane to dry
2. Assemble Electrophoresis Module (see Mini-Protean 3 Cell assembly guide) 3. Sample preparation: a.Total volume to be loaded: 30ul x ml of sample (use 30ug total protein/well) 5 ml 6x Leammlis sample buffer y ml dH20 (for a total
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